MES is widely used as a buffering agent in biochemical and molecular biology applications due to its effective pH range of 5.5 to 6.7. It is particularly useful in cell culture media, electrophoresis, and protein purification processes where maintaining a stable pH is critical. In electrophoresis, MES helps ensure consistent separation of proteins and nucleic acids. It is also employed in chromatography as a mobile phase buffer to enhance the resolution of biomolecules. Additionally, MES is utilized in enzyme assays and diagnostic kits where precise pH control is necessary for optimal activity and accuracy. Its low ultraviolet absorbance makes it suitable for applications involving spectrophotometric analysis.