MES is widely used as a buffering agent in biochemical and molecular biology applications due to its effective pH range of 5.5 to 6.7. It is commonly employed in cell culture media to maintain a stable environment for cell growth and viability. Additionally, MES is utilized in electrophoresis, particularly in SDS-PAGE, to ensure consistent pH levels during protein separation. Its low UV absorbance makes it suitable for use in spectrophotometric assays and chromatography. MES is also applied in plant tissue culture to optimize nutrient uptake and support healthy plant development. Furthermore, it serves as a buffer in enzyme assays and protein purification processes, ensuring optimal activity and stability of biological molecules.