Glycylglycine buffer is widely used in biochemical and biophysical research due to its effective buffering capacity in the pH range of 7.5 to 8.5. It is particularly useful in enzyme assays, protein studies, and cell culture applications where maintaining a stable pH is critical. The buffer is also employed in the preparation of electrophoresis gels, as it helps maintain the optimal pH for protein separation. Additionally, glycylglycine buffer is utilized in the study of enzyme kinetics and protein-protein interactions, as it minimizes interference with biological processes. Its compatibility with various biochemical reagents makes it a preferred choice in laboratory settings.