MES is widely used as a buffering agent in biochemical and biological research. It is particularly effective in maintaining stable pH conditions in cell cultures and enzyme assays, ensuring optimal activity and stability of biological molecules. Its low absorbance in the UV range makes it suitable for spectrophotometric studies, where it does not interfere with measurements. Additionally, MES is commonly employed in electrophoresis and chromatography techniques to provide consistent pH environments, enhancing the separation and analysis of proteins and nucleic acids. Its compatibility with various biochemical processes and minimal interaction with metal ions further contribute to its utility in laboratory applications.