Glycine buffer in PBS is widely used in biochemical and molecular biology applications due to its effective buffering capacity in the pH range of 2.0–3.6 and 8.6–10.6. It is commonly employed in protein purification processes, particularly in affinity chromatography, where it helps elute antibodies or other proteins bound to resins. The buffer is also utilized in electrophoresis techniques, such as SDS-PAGE, to maintain stable pH conditions during protein separation. In cell culture, it serves as a stabilizing agent for maintaining the integrity of cells and biomolecules. Additionally, it is used in immunoassays, including ELISA, to minimize non-specific binding and improve assay sensitivity. Its compatibility with PBS enhances its utility in various biological research and diagnostic applications.