Glycine buffer in PBS is widely used in biological and biochemical research due to its ability to maintain a stable pH environment. It is commonly employed in protein purification processes, particularly during ion exchange and affinity chromatography, where it helps to elute proteins without causing denaturation. In immunoassays, such as ELISA, it serves as a blocking agent to prevent non-specific binding of antibodies, enhancing the accuracy of results. Additionally, it is utilized in cell culture applications to maintain optimal pH conditions for cell growth and viability. Glycine buffer in PBS is also a key component in electrophoresis, where it helps to stabilize the pH of the running buffer, ensuring consistent separation of proteins or nucleic acids. Its buffering capacity and compatibility with biological systems make it a versatile tool in various laboratory protocols.