Glycine buffer in PBS is widely used in biological and biochemical research due to its ability to maintain a stable pH environment. It is commonly employed in protein purification processes, particularly in affinity chromatography, where it helps in the elution of antibodies and other proteins from columns. The buffer is also utilized in immunoassays, such as ELISA, to wash and block non-specific binding sites, ensuring accurate and reliable results. In cell culture applications, glycine buffer in PBS aids in maintaining optimal conditions for cell growth and viability. Additionally, it is used in electrophoresis techniques, including SDS-PAGE, to prepare samples and running buffers, ensuring consistent separation of proteins. Its compatibility with biological systems makes it a valuable tool in various laboratory procedures.