Glycine buffer in PBS is widely used in biochemical and molecular biology applications due to its effective buffering capacity at physiological pH ranges. It is commonly employed in protein and nucleic acid electrophoresis to maintain stable pH conditions during separation processes. The buffer is also utilized in immunoassays, such as ELISA, to minimize non-specific binding and enhance the specificity of antibody-antigen interactions. Additionally, it is used in cell culture applications to maintain optimal pH levels for cell growth and viability. In protein purification, glycine buffer in PBS aids in elution and stabilization of proteins during chromatography. Its compatibility with biological systems makes it a preferred choice for various laboratory procedures requiring precise pH control.