Tris(hydroxymethyl)aminomethane acetate salt is widely used as a buffering agent in biochemical and molecular biology applications. It helps maintain a stable pH in various experimental setups, such as electrophoresis, particularly for DNA and RNA analysis. The compound is also employed in cell culture media to ensure optimal growth conditions for cells by stabilizing the pH. Additionally, it is utilized in protein purification processes and as a component in diagnostic assays. Its buffering capacity makes it suitable for use in enzyme studies and other biochemical reactions where pH control is critical.