Tris(hydroxymethyl)aminomethane hydrochloride (Tris HCl)
Reagent grade, ≥99%
Reagent
Code: #97712
Alias
2-Amino-2-(hydroxymethyl)-1,3-propanediol ; Tris-(hydroxymethyl)aminomethane hydrochloride;
CAS Number
1185-53-1
blur_circular Chemical Specifications
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Molecular Information
Weight
157.6 g/mol
Formula
C₄H₁₂ClNO₃
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Registry Numbers
EC Number
214-684-5
MDL Number
MFCD00012590
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Physical Properties
Melting Point
150-152 °C
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Storage & Handling
Density
1.05 g/mL at 20 °C
Storage
room temperature, dry
description Product Description
Tris HCl is widely used as a buffering agent in biochemical and molecular biology applications. It helps maintain a stable pH in various solutions, particularly in electrophoresis and chromatography techniques. In DNA and RNA extraction, it ensures optimal conditions for enzymatic reactions. It is also employed in cell culture media to regulate pH and support cell growth. Additionally, Tris HCl is utilized in protein purification processes to stabilize proteins and maintain their functionality. Its effectiveness in maintaining pH makes it a key component in diagnostic assays and pharmaceutical formulations.
format_list_bulleted Product Specification
| Test Parameter | Specification |
|---|---|
| Melting Point (°C) | 151-156 |
| Purity | 99-100% |
| Appearance | White powder or crystals |
| pH (1.0 mol/L) | 4.2-5.2 |
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