Tris Buffered Saline (TBS) is widely used in biological and biochemical research as a reliable buffer solution. It is commonly employed to maintain a stable pH environment during experiments, particularly in immunohistochemistry and immunocytochemistry. TBS is often used as a washing buffer to rinse samples and remove unbound antibodies or other reagents, ensuring clean and accurate results. It is also utilized in Western blotting procedures to dilute antibodies and wash membranes, reducing background noise and enhancing specificity. Additionally, TBS serves as a diluent for various proteins and enzymes, maintaining their stability and activity. Its compatibility with biological tissues and minimal interference with molecular interactions make it a preferred choice in cell culture and molecular biology applications.