Tris Buffered Saline (TBS) is widely used in biological and biochemical research as a reliable buffer solution. It is commonly employed in immunoassays, such as Western blotting and ELISA, to wash membranes and dilute antibodies, ensuring optimal binding conditions. TBS is also utilized in immunohistochemistry for tissue section washing, as it maintains pH stability and prevents nonspecific binding. In cell culture, it serves as a rinsing agent to remove residual media or contaminants. Additionally, TBS is often combined with detergents like Tween-20 to enhance washing efficiency in protein studies. Its compatibility with various biological molecules makes it a versatile tool in molecular biology and diagnostic applications.