Tris Buffered Saline (TBS, 10x, low salt) is widely used in biological and biochemical research for its ability to maintain a stable pH environment. It is commonly employed as a washing buffer in immunoassays, such as ELISA and Western blotting, to remove unbound antibodies or other reagents, ensuring clean and specific results. In immunohistochemistry, TBS is used to dilute antibodies and wash tissue sections, minimizing background staining and improving clarity. It is also utilized in cell culture applications to rinse cells gently without causing osmotic stress. Additionally, TBS serves as a diluent for various reagents and as a buffer in molecular biology techniques, including DNA and protein hybridization. Its low salt concentration makes it particularly suitable for applications where minimizing ionic interactions is critical.