TES is widely used as a buffering agent in biochemical and molecular biology research, effective in the pH range of 6.8-8.2. It is particularly effective in maintaining stable pH levels in cell culture media, enzyme assays, and protein purification processes. TES is often chosen for its compatibility with biological systems and its minimal interference with biochemical reactions. It is also utilized in electrophoresis techniques, where it helps to maintain consistent pH conditions during the separation of proteins and nucleic acids. Additionally, TES is employed in the preparation of buffers for studying enzyme kinetics and in the stabilization of sensitive biological samples. Its low metal ion binding capacity makes it suitable for applications requiring minimal metal interference, such as in studies involving metal-sensitive enzymes or proteins.