Tricine buffer is widely used in biochemical and molecular biology research due to its effective buffering range in the pH 8.0–8.8 range. It is particularly useful in SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) for separating small proteins and peptides, as it provides better resolution compared to other buffers. Additionally, Tricine buffer is employed in enzyme assays, particularly for enzymes that function optimally in slightly alkaline conditions. It is also utilized in the preparation of samples for mass spectrometry and in the study of protein-protein interactions. Its low ionic strength and minimal interference with biochemical reactions make it a preferred choice in various laboratory applications.