POPSO buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 7.2 to 8.5. It is particularly useful in cell culture applications, where maintaining a stable pH is crucial for cell growth and viability. Additionally, POPSO buffer is employed in enzyme assays and protein purification processes, as it helps maintain optimal conditions for enzymatic activity and protein stability. Its compatibility with various biological systems makes it a reliable choice for experiments involving DNA and RNA manipulation, ensuring consistent and reproducible results.