PIPES Buffered Saline is widely used in biological and biochemical research as a stabilizing agent for maintaining pH in cell culture media and biochemical assays. Its effective buffering range makes it suitable for experiments involving enzymes, proteins, and other sensitive biological molecules, ensuring optimal conditions for their activity and stability. It is also commonly employed in immunology and cell biology studies, particularly for washing and resuspending cells during flow cytometry and immunofluorescence procedures. Additionally, PIPES Buffered Saline is utilized in electrophoresis and chromatography techniques, where consistent pH control is critical for accurate results. Its low toxicity and compatibility with various biological systems further enhance its utility in laboratory applications.