PIPES Buffered Saline (effective pH range 6.1-7.5) is widely used in biological and biochemical research as a stabilizing agent for maintaining pH in cell cultures and enzymatic reactions. It is particularly effective in experiments involving proteins and nucleic acids, where precise pH control is critical. The buffer is also utilized in microscopy and immunohistochemistry to preserve cell structure and integrity during staining procedures. Additionally, it serves as a reliable medium for washing and resuspending cells in flow cytometry and other cell-based assays. Its low reactivity with metal ions makes it suitable for studies involving metal-sensitive enzymes or processes.