Phosphate Buffered Saline with Tween 20 is widely used in biological and biochemical research. It serves as a buffer solution to maintain a stable pH environment, which is crucial for various laboratory procedures. The inclusion of Tween 20, a non-ionic detergent, enhances its utility by reducing surface tension and preventing non-specific binding of proteins in immunoassays such as ELISA and Western blotting. This makes it an essential component in the preparation and washing of samples, ensuring accurate and reliable results. Additionally, it is employed in cell culture applications to rinse cells without causing damage, maintaining cell integrity during experiments. Its versatility and effectiveness make it a staple in laboratories focusing on molecular biology, immunology, and cell biology.