PBS Buffer, pH 5.0, is widely used in biological and biochemical research for maintaining a stable pH environment in various experimental procedures. It is particularly useful in immunoassays, such as ELISA, where it helps in the dilution of antibodies and antigens, ensuring optimal binding conditions. This buffer is also employed in cell culture applications to wash cells and maintain their physiological pH during experiments. In molecular biology, it is utilized for DNA and RNA hybridization processes, where a slightly acidic pH is required. Additionally, PBS Buffer, pH 5.0, is used in protein purification and stabilization, as it prevents denaturation and maintains protein integrity. Its consistent pH level makes it a reliable choice for various laboratory protocols requiring precise pH control.