MOPS buffer is widely used in biological and biochemical research due to its effective buffering capacity in the pH range of 6.5 to 7.9. It is commonly employed in cell culture media to maintain a stable pH environment, ensuring optimal growth conditions for cells. In molecular biology, MOPS is frequently utilized in gel electrophoresis, particularly for RNA separation, as it provides consistent and reliable results. Additionally, it is used in protein purification and enzyme assays to maintain pH stability during experimental procedures. MOPS buffer is also applied in plant tissue culture and microbial studies, where precise pH control is critical for successful outcomes. Its low reactivity with biological molecules makes it a preferred choice in various laboratory applications.