MOPS buffer is widely used in biological and biochemical research due to its effective buffering capacity in the pH range of 6.5 to 7.9. It is commonly employed in cell culture media to maintain stable pH conditions, ensuring optimal growth and viability of cells. In molecular biology, MOPS is frequently used in electrophoresis, particularly for RNA separation in agarose gels, as it provides a stable environment that minimizes RNA degradation. Additionally, it is utilized in protein purification processes and enzyme assays where maintaining a consistent pH is critical for accurate results. Its low reactivity with metal ions also makes it suitable for use in studies involving metal-dependent enzymes. Overall, MOPS buffer is a versatile and reliable choice for various laboratory applications requiring precise pH control.