MOPSO buffer is widely used in biological and biochemical research due to its effective pH stabilization in the range of 6.5 to 7.9. It is commonly employed in cell culture media to maintain a consistent pH environment, which is crucial for cell growth and viability. Additionally, MOPSO buffer is utilized in electrophoresis, particularly for RNA separation, as it provides stable pH conditions that are essential for accurate results. It is also applied in enzyme assays and protein purification processes, where maintaining a specific pH is critical for optimal enzyme activity and protein stability. Furthermore, MOPSO buffer is used in various molecular biology techniques, including nucleic acid hybridization and chromatography, ensuring reliable and reproducible outcomes. Its low toxicity and compatibility with biological systems make it a preferred choice in many laboratory applications.