MES buffer is widely used in biochemical and molecular biology research due to its effective buffering range of pH 5.5 to 6.7. It is commonly employed in electrophoresis, particularly for separating nucleic acids and proteins, as it provides stable pH conditions during the process. MES is also utilized in cell culture applications to maintain optimal pH levels for cell growth and viability. Additionally, it serves as a buffer in chromatography techniques, including ion-exchange and affinity chromatography, to ensure consistent separation and purification of biomolecules. In enzyme assays, MES helps maintain the necessary pH for accurate enzyme activity measurements. Its low UV absorbance makes it suitable for spectrophotometric analyses, minimizing interference with detection.