This 0.2 M MOPS buffer solution, adjusted to pH 7.0, is widely used in biological and biochemical research due to its effective buffering capacity in the pH range of 6.5 to 7.9. It is commonly employed in molecular biology techniques, particularly in RNA electrophoresis, where it helps maintain a stable pH environment, ensuring accurate separation and analysis of RNA samples. Additionally, MOPS Buffer is utilized in cell culture applications to maintain optimal pH conditions for cell growth and viability. It is also a preferred buffer in protein purification processes and enzyme assays, where consistent pH is critical for maintaining protein stability and activity. Its low UV absorbance makes it suitable for use in spectrophotometric analyses without interfering with measurements.