MES buffer is widely used in biochemical and molecular biology research due to its effective buffering range of pH 5.5 to 6.7. It is commonly employed in electrophoresis, particularly for separating proteins and nucleic acids, as it provides stable pH conditions during the process. MES buffer is also utilized in cell culture applications to maintain optimal pH levels for cell growth and viability. Additionally, it serves as a buffering agent in chromatography techniques, ensuring consistent separation and analysis of biomolecules. Its low UV absorbance makes it suitable for spectrophotometric assays and other analytical procedures where minimal interference is required. MES buffer is also used in enzyme studies, protein purification, and molecular techniques such as PCR, where precise pH control is critical for maintaining activity and stability.