HEPPS buffer is widely used in biochemical and biophysical research due to its effective buffering capacity in the pH range of 7.3 to 8.7. It is particularly valuable in cell culture applications, where it helps maintain a stable pH environment, ensuring optimal growth conditions for cells. In protein studies, HEPPS is often utilized for protein purification and crystallization processes, as it provides a consistent pH without interfering with protein stability. Additionally, it is employed in electrophoresis and chromatography techniques to maintain pH stability during separation and analysis of biomolecules. Its low metal ion contamination makes it suitable for sensitive enzymatic assays and other applications where metal ions could interfere. HEPPS is also used in molecular biology for DNA and RNA hybridization experiments, where precise pH control is critical for accurate results.