HEPPSO buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 7.0 to 8.0. It is particularly valuable in studies involving enzymes, proteins, and nucleic acids, where maintaining a stable pH is critical for optimal activity and stability. HEPPSO is often employed in cell culture media, electrophoresis, and chromatography applications, ensuring consistent experimental conditions. Additionally, it is utilized in the preparation of buffers for assays and diagnostic kits, where precise pH control is essential. Its low toxicity and compatibility with biological systems make it a preferred choice for in vitro studies and biotechnological processes.