HEPPSO buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 7.0 to 8.2. It is particularly useful in cell culture applications, where maintaining a stable pH is critical for cell growth and viability. HEPPSO is also employed in enzyme studies and protein purification processes, as it helps maintain optimal pH conditions without interfering with enzymatic activity or protein stability. Additionally, it is utilized in electrophoresis and chromatography techniques to ensure consistent pH levels during separation and analysis of biomolecules. Its low metal ion binding capacity makes it suitable for experiments involving metal-sensitive proteins or enzymes. Overall, HEPPSO buffer is a reliable choice for maintaining pH stability in various biological and biochemical applications.