HEPPSO buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 7.0 to 8.2. It is particularly useful in cell culture applications, where maintaining a stable pH is critical for cell growth and viability. HEPPSO is also employed in protein purification processes, as it helps maintain the stability and functionality of proteins during extraction and purification steps. Additionally, it is utilized in enzyme assays and other biochemical experiments where precise pH control is essential for accurate results. Its low metal ion contamination makes it suitable for sensitive applications, such as trace metal analysis and studies involving metal-dependent enzymes.