HEPES buffer is widely used in biological and biochemical research due to its ability to maintain a stable pH in cell culture media and enzymatic reactions. It is particularly favored in cell and tissue culture applications because it does not interfere with cell growth and remains effective across a range of temperatures. HEPES is also commonly employed in protein studies, electrophoresis, and chromatography, where consistent pH levels are crucial for accurate results. Additionally, it is used in molecular biology techniques, such as DNA and RNA isolation, to ensure optimal conditions for enzymatic activity. Its non-toxic nature and compatibility with various biological systems make it a reliable choice for maintaining pH stability in sensitive experiments.