DIPSO buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 7.0 to 8.2. It is particularly valuable in cell culture applications, where maintaining a stable pH is crucial for cell growth and viability. DIPSO is also employed in protein purification processes, as it helps maintain the stability and functionality of proteins during extraction and separation. Additionally, it is utilized in enzymatic assays to ensure optimal activity of enzymes that are sensitive to pH fluctuations. Its compatibility with biological systems makes it a preferred choice for various in vitro studies, including those involving nucleic acids and other biomolecules.