DIPSO buffer is widely used in biochemical and molecular biology research for maintaining stable pH conditions in various experimental setups. It is particularly effective in cell culture applications, where it helps to provide a consistent environment for cell growth and viability. The buffer is also utilized in protein studies, including purification and crystallization processes, due to its ability to maintain pH without interfering with protein structure or function. Additionally, DIPSO buffer finds application in enzymatic assays, where precise pH control is crucial for accurate activity measurements. Its compatibility with biological systems makes it a reliable choice for maintaining pH in sensitive biochemical reactions.