CAPS buffer is widely used in biochemical and molecular biology applications, particularly for protein and nucleic acid electrophoresis. Its effective buffering range between pH 9.7 and 11.0 makes it suitable for alkaline pH conditions, which are often required in capillary electrophoresis and western blotting. CAPS buffer is commonly employed in the transfer of proteins from polyacrylamide gels to membrane supports during western blotting, ensuring efficient and high-resolution protein transfer. Additionally, it is utilized in the purification and crystallization of proteins, as well as in enzymatic assays that require a stable alkaline environment. Its compatibility with various biochemical techniques and its ability to maintain pH stability make CAPS buffer a valuable tool in laboratory research.