CAPS buffer is widely used in biochemical and molecular biology applications due to its effective buffering range at higher pH levels, typically between 9.7 and 11.1. It is commonly employed in protein electrophoresis, particularly in SDS-PAGE, where it helps maintain a stable pH environment for separating proteins based on their molecular weights. Additionally, CAPS buffer is utilized in Western blotting as a transfer buffer, facilitating the efficient movement of proteins from gels to membranes. Its compatibility with mass spectrometry also makes it suitable for protein analysis and peptide mapping. Furthermore, CAPS buffer is used in various enzymatic assays and cell culture applications where a higher pH environment is required. Its stability and minimal interference with biological processes make it a reliable choice for these techniques.