CAPSO buffer is widely used in biochemical and molecular biology research due to its effective pH range of 8.9–10.3, making it suitable for alkaline conditions. It is commonly employed in enzyme studies, particularly for enzymes that function optimally at higher pH levels, such as alkaline phosphatases. CAPSO buffer is also utilized in protein purification processes, including chromatography, where maintaining a stable pH is critical. Additionally, it is applied in cell culture experiments to maintain physiological conditions and in diagnostic assays to ensure accurate results. Its low metal ion contamination makes it ideal for sensitive applications requiring minimal interference.