Bis-Tris buffer (0.2 M, pH 6.0) is widely used in biochemical and molecular biology research due to its effective buffering range (typically 6.0-7.5) and compatibility with various biological systems. It is commonly employed in protein purification processes, particularly in chromatography techniques, where it helps maintain stable pH conditions. Bis-Tris is also utilized in gel electrophoresis, especially for separating proteins and nucleic acids, as it provides consistent pH and minimizes protein degradation. Additionally, it is suitable for enzyme assays and cell culture applications, ensuring optimal conditions for enzymatic activity and cell growth. Its low reactivity with metal ions makes it a preferred choice in studies involving metal-dependent enzymes or proteins.