Bis-Tris Propane Buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity across a broad pH range, typically between 6.3 and 9.5. It is particularly valuable in protein purification and crystallization processes, where maintaining a stable pH is critical for preserving protein structure and function. Additionally, it is employed in enzymatic assays and electrophoresis, as it minimizes interference with enzymatic activity and provides consistent results. Its low metal ion contamination makes it suitable for studies involving metal-sensitive proteins or enzymes. Bis-Tris Propane Buffer is also utilized in cell culture applications to maintain optimal pH conditions for cell growth and viability. Its versatility and reliability make it a preferred choice in various laboratory settings.