Bicine buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 7.6 to 9.0. It is particularly useful in enzyme assays, protein purification, and electrophoresis, where maintaining a stable pH is crucial. Bicine is also employed in cell culture media to support optimal growth conditions for certain cell types. Additionally, it is utilized in the preparation of reagents for nucleic acid studies and in the crystallization of proteins, where precise pH control is essential. Its low metal ion chelating properties make it suitable for applications involving metal-sensitive enzymes or proteins.