Bis-Tris Buffer is widely used in biochemical and molecular biology research for maintaining stable pH conditions in various experimental procedures. It is particularly effective in the pH range of 5.8 to 7.2, making it suitable for applications such as protein electrophoresis, enzyme assays, and cell culture media preparation. Its low metal ion contamination ensures minimal interference with sensitive biochemical reactions. Additionally, Bis-Tris Buffer is often employed in chromatography and crystallization processes to stabilize proteins and nucleic acids. Its compatibility with a wide range of biological systems and its ability to resist pH changes make it a reliable choice for maintaining optimal experimental conditions.