BES buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 6.4 to 7.8. It is commonly employed in cell culture applications, where it helps maintain a stable pH environment essential for cell growth and viability. BES buffer is also utilized in protein and nucleic acid research, particularly in electrophoresis and chromatography techniques, where it ensures consistent pH conditions for accurate results. Additionally, it is a key component in transfection experiments, where it assists in the efficient delivery of genetic material into cells. Its low toxicity and compatibility with various biological systems make it a preferred choice in laboratory settings.