Bis-Tris buffer is widely used in biochemical and molecular biology research due to its effective buffering range between pH 5.8 and 7.2. It is particularly useful in protein and nucleic acid studies, as it stabilizes pH without interfering with enzymatic reactions or protein functions. Bis-Tris is commonly employed in gel electrophoresis, especially for resolving low molecular weight proteins, as it provides better resolution and sharper bands compared to other buffers. It is also used in chromatography and cell culture applications to maintain optimal pH conditions. Additionally, Bis-Tris is preferred in crystallization experiments for proteins and in the preparation of vaccines, where maintaining a stable pH is critical for preserving the integrity of biological molecules. Its low toxicity and compatibility with various biochemical assays make it a versatile choice in laboratory settings.