BES Buffer is widely used in biochemical and molecular biology applications due to its effective buffering range of pH 6.4 to 7.8. It is commonly employed in cell culture media to maintain a stable pH environment, which is crucial for the growth and viability of cells. Additionally, BES Buffer is utilized in protein purification processes, particularly in chromatography, where it helps maintain the stability and functionality of proteins. It is also a key component in transfection experiments, where it assists in the efficient delivery of nucleic acids into cells. Its low toxicity and compatibility with biological systems make it a preferred choice in various laboratory protocols.