AMPD Buffer (0.2M, pH 8.5) is widely used in biochemical and molecular biology applications due to its effective buffering capacity at a slightly alkaline pH. It is commonly employed in protein purification processes, particularly in ion-exchange chromatography, where it helps maintain a stable pH environment to ensure optimal protein stability and activity. The buffer is also utilized in enzyme assays and kinetic studies, as it provides a consistent pH that is suitable for many enzymatic reactions. Additionally, AMPD Buffer is used in electrophoresis techniques, such as SDS-PAGE, to maintain the pH of the running buffer, ensuring accurate separation of proteins. Its application extends to cell culture media preparation, where it helps stabilize the pH during cell growth and experimentation.