ADA buffer is widely used in biochemical and molecular biology applications due to its effective buffering capacity in the pH range of 6.0 to 7.2. It is commonly employed in enzyme assays, protein purification, and cell culture media to maintain a stable pH environment, ensuring optimal activity and stability of biological molecules. Additionally, ADA buffer is utilized in electrophoresis techniques, such as SDS-PAGE, to provide consistent pH conditions during protein separation. Its compatibility with various biological systems makes it a reliable choice for research and diagnostic procedures requiring precise pH control.