ACES buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 6.1 to 7.5. It is particularly favored in cell culture applications, where it helps maintain a stable pH environment, ensuring optimal conditions for cell growth and viability. Additionally, ACES buffer is commonly employed in protein purification processes, electrophoresis, and enzyme assays, where precise pH control is critical for maintaining protein stability and activity. Its low UV absorbance makes it suitable for use in spectrophotometric analyses, minimizing interference with measurements. ACES buffer is also utilized in diagnostic assays and as a component in various biological and chemical research protocols.