ACES buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 6.1 to 7.5. It is particularly favored in cell culture applications, where it helps maintain a stable pH environment, ensuring optimal growth conditions for cells. Additionally, ACES buffer is utilized in electrophoresis techniques, such as SDS-PAGE, to provide consistent pH levels during protein separation. It is also employed in enzyme assays and other biochemical experiments where precise pH control is critical for accurate results. Its compatibility with various biological systems and minimal interference with enzymatic activities make it a reliable choice in laboratory settings.