DIPSO is commonly used as a buffering agent in biochemical and molecular biology research. It helps maintain a stable pH in cell culture media, enzyme assays, and protein purification processes. Its effectiveness in the pH range of 7.0 to 8.2 makes it suitable for studies involving biological systems that require neutral to slightly alkaline conditions. Additionally, DIPSO is often employed in electrophoresis and chromatography techniques to ensure consistent experimental conditions. Its low toxicity and compatibility with various biological materials further enhance its utility in laboratory applications.