MOPS is widely used as a buffering agent in biological and biochemical research. It is particularly effective in maintaining stable pH conditions in cell culture media, electrophoresis, and protein purification processes. Its zwitterionic nature makes it suitable for use in a variety of experimental setups, including those involving enzymes and nucleic acids, where it helps to minimize interference with biological activity. Additionally, MOPS is often employed in molecular biology techniques such as Northern blotting and RNA electrophoresis, providing a consistent environment that supports accurate and reproducible results. Its low toxicity and compatibility with many biological systems further enhance its utility in laboratory applications.