HEPPS is widely used as a buffering agent in biochemical and molecular biology research. It is particularly effective in maintaining stable pH conditions in cell culture media, enzyme assays, and protein purification processes. Its buffering range, typically between pH 7.0 and 8.5, makes it suitable for applications requiring near-physiological pH levels. HEPPS is also utilized in electrophoresis and chromatography techniques to ensure consistent and reliable results. Additionally, it is employed in studies involving membrane proteins and other sensitive biological systems due to its minimal interference with biological processes. Its stability and low toxicity further enhance its utility in various laboratory applications.